成大生化所
余建泓 助理教授

Assistant Professor Chien-Hung Yu

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通訊地址： 701 台南市大學路一號
連絡分機： 5540(辦公室) 5525(實驗室)
E-mail： chienhung_yu@mail.ncku.edu.tw

學歷
荷蘭萊頓大學 博士 (2011)

專長領域
● 分子生物學
● 蛋白質轉譯
● 核醣核酸生物學

研究興趣
密碼子是將遺傳訊息傳遞到胺基酸以至於蛋白質的橋樑，也是分子生物學的中心法則的核心。絕大部分的胺基酸可以被兩個以上的同義密碼子表達，但是這些同義密碼子的使用頻率並不相同，我們稱這種現象為密碼子使用偏移。有趣的是，密碼子使用偏移存在於幾乎所有已知的基因體中，但是為什麼這種現象會在長久的演化過程中被保留下來，並不清楚。 我們之前的研究結果顯示，密碼子的使用偏移雖然不改變胺基酸的序列，卻可以調控蛋白質的摺疊以及調控信使核醣核酸與蛋白質的表達量。在臨床上，隨著定序技術的進步，越來越多的疾病也被發現是與序列中同義密碼子的突變有關。未來實驗室的研究將運用分子生物學、生物化學、遺傳學以及生物資訊學的方法，來探討為什麼密碼子使用偏移會影響基因表達的機制。也希望透過更深入了解密碼子使用偏移的機制，可以發展出治療同義密碼子突變相關疾病的方法。

Publications:

  1. Ku, Y. C., Lin, P. H., Huang, C. Y., Lee, C. W., Yu, C.H., Chen, S. C. & Liu, W. M., (2023) An iminocoumarin based covalent-assembly red-emitting fluorescent probe for detection of β-galactosidase activity in ovarian cancer cells. Dyes and Pigments. 210, 111004.

  2. Chen, S. Y., Chen, K. L., Ding, L. Y., Yu, C.H., Wu, H. Y., Chou, Y. Y., Chang, C. J., Chang, C. H., Wu, Y. N., Wu, S. R., Hou, Y. C., Lee, C. T., Chen, P. C., Shan, Y. S. & Huang, P. H., (2022) RNA bisulfite sequencing reveals NSUN2-mediated suppression of epithelial differentiation in pancreatic cancer Oncogene. 41, 22, p. 3162-3176 15 p. [Abstract]

  3. Yang, T. H., Hsu, C. W., Wang, Y. X., Yu, C.H., Jagat, R., Tseng, Y. Y. & Wu, W. S., (2022) YMLA: A comparative platform to carry out functional enrichment analysis for multiple gene lists in yeast. Computers in Biology and Medicine. 151, 106314. [Abstract]

  4. Chen, S. C., Olsthoorn, R. C. L. and Yu, C.H., (2021) Structural phylogenetic analysis reveals lineage-specific RNA repetitive structural motifs in all coronaviruses and associated variations in SARS-CoV-2. Virus Evolution. 7, 1, veab021. [Abstract]

  5. Yang, Q., Yu, C.H., Zhao, F., Dang, Y., Wu, C., Xie, P., Sachs, M. S. and Liu, Y., (2019) eRF1 mediates codon usage effects on mRNA translation efficiency through premature termination at rare codons. Nucleic acids research. 47, 17, p. 9243-9258 16 p. [Abstract]

  6. Zhao, F., Yu, C.H., and Liu, Y., (2017) Codon usage regulates protein structure and function by affecting translation elongation speed in Drosophila cells Nucleic acids research. 45, 14, p. 8484-8492 9 p. [Abstract]

  7. Zhou, Z., Dang, Y., Zhou, M., Li, L., Yu, C.H., Fu, J., Chen, S., and Liu, Y., (2016) Codon usage is an important determinant of gene expression levels largely through its effects on transcription. Proc. Natl. Acad. Sci. U.S.A., 113, E6117-E6125. [Abstract]

  8. Yu, C.H.*, Dang, Y.*, Zhou, Z*., Wu, C., Zhao, F., Sachs, M.S., and Liu, Y., (2015) Codon usage influences the local rate of translation elongation to regulate co-translational protein folding. Mol. Cell, 59, 744-754. (*co-first authors) (Cover article and highlighted in TIBS) [Abstract]

  9. Yu, C.H. and Olsthoorn, R.C., (2015) Monitoring ribosomal frameshifting as a platform to screen anti-riboswitch drug candidates. Methods Enzymol.,550, 385-393. [Abstract]

  10. Yu, C.H. and Olsthoorn, R.C., (2014) Stimulation of ribosomal frameshifting by RNA G-quadruplex structures. Nucleic Acids Res., 42, 1887-1892. [Abstract]

  11. Luo, J., Wärmländer, S.K., Yu, C.H., Muhammad, K., Gräslund, A., and Abrahams, J.P., (2014) The Aβ peptide forms non-amyloid fibrils in the presence of carbon nanotubes. Nanoscale., 6(12):6720-6. [Abstract]

  12. Luo, J., Otero, J.M., Yu, C.H., Wärmländer, S.K., Gräslund, A., Overhand, M., and Abrahams, J.P., (2013) Inhibiting and reversing amyloid-β peptide (1-40) fibril formation with gramicidin S and engineered analogues. Chemistry, 19, 17338-17348. [Abstract]

  13. Luo, J., Yu, C.H., Yu, H., Borstnar, R., Kamerlin, S.C., Gräslund, A., Abrahams, J.P., and Wärmländer, S.K., (2013) Cellular polyamines promote amyloid-beta (Aβ) peptide fibrillation and modulate the aggregation pathways. ACS Chem. Neurosci., 4, 454-62. [Abstract]

  14. Yu, C.H. Luo J., Iwata-Reuyl, D., and Olsthoorn, R.C., (2013) Exploiting preQ1 riboswitches to regulate ribosomal frameshifting. ACS Chem. Biol., 8, 733-740. [Abstract]

  15. Yu, C.H., Noteborn, M.H., Pleij, C.W., and Olsthoorn, R.C., (2011) Stem-loop structures can effectively substitute for an RNA pseudoknot in -1 ribosomal frameshifting. Nucleic Acids Res., 39, 8952-8959. [Abstract]

  16. Yu, C.H., Noteborn, M.H., and Olsthoorn, R.C., (2010) Stimulation of ribosomal frameshifting by antisense LNA. Nucleic Acids Res., 38, 8277-8283. [Abstract]

  17. Lien, H.Y., Yu, C.H., Liou, C.M., and Wu, W.F., (2009) Regulation of clpQY (hslVU) Gene Expression in Escherichia coli. Open Microbiol. J., 3, 29-39. [Abstract]

  18. Lee, Y.Y., Chang, C.F., Kuo, C.L., Chen, M.C., Yu, C.H., Lin, P.I., and Wu, W.F., (2003) Subunit oligomerization and substrate recognition of the Escherichia coli ClpYQ (HslUV) protease implicated by in vivo protein-protein interactions in the yeast two-hybrid system. J. Bacteriol., 185, 2393-2401. [Abstract]