Hua-Lin Wu

Chair Professor

Prof. Hua-Lin Wu

The HL Wu's laboratory is interested in studying the biological functions of thrombomodulin (TM) and angiostatin. We have found that TM not only serves as an anti-coagulant, but also mediates cell-to-cell adhesion. For investigating the novel functions of TM, we have constructed various DNA fragments of TM gene and generated cell clones that stably expressed these genes. The cell morphology, TM distribution and cell-cell interaction of these cells were then analyzed and the biological functions of the TM domains were evaluated. We also assess the mechanisms of TM-mediated tumor cell proliferation, migration and signal transduction. Recently, we have cloned genes that are highly homologous to TM, including endosialin and C1qRP. The functions of both proteins will be studied further. In the study of angiostatin, we utilized genetic engineering, protein expression and purification methods to prepare several angiostatin mutant proteins. In order to find the most effective protein, the inhibitory effects of these proteins on endothelial cell proliferation, migration and angiogenesis were analyzed in cell culture and animal models. In addition, the correlation of gene polymorphisms, gene expressions and atherosclerosis is currently studied.

A wide variety of experimental techniques and procedures are used in Wu's laboratory, such as gene cloning, site-directed mutagenesis, bacterial and yeast transformation, large scale culture by fermentation, protein expression and purification, cell culture, fluorescent confocal microscopy, flow cytometry, angiogenic Matrigel plug assay in mice. Consequently many different types of projects could be developed depending on the discussion between students and Professor Wu in the future.

Publications:

  1. Chao TH, Tsai WC, Chen JY, Liu PY, Chung HC, Tseng SY, Kuo CH, Shi GY, Wu HL, Li YH. Soluble thrombomodulin is a paracrine anti-apoptotic factor for vascular endothelial protection. Int J Cardiol. 2014 [Abstract]

  2. Chao TH, Tseng SY, Chen IC, Tsai YS, Huang YY, Liu PY, Ou HY, Li YH, Wu HL, Cho CL, Tsai LM, Chen JH. Cilostazol enhances mobilization and proliferation of endothelial progenitor cells and collateral formation by modifying vasculo-angiogenic biomarkers in peripheral arterial disease. Int J Cardiol. 2014 [Abstract]

  3. Cheng TL, Lai CH, Jiang SJ, Hung JH, Liu SK, Chang BI, Shi GY, Wu HL. RHBDL2 Is a Critical Membrane Protease for Anoikis Resistance in Human Malignant Epithelial Cells. ScientificWorldJournal. 2014 [Abstract]

  4. Lai CH, Shi GY, Lee FT, Kuo CH, Cheng TL, Chang BI, Ma CY, Hsu FC, Yang YJ, Wu HL. Recombinant human thrombomodulin suppresses experimental abdominal aortic aneurysms induced by calcium chloride in mice. Ann Surg. 2013 [Abstract]

  5. Chen PK, Chang BI, Kuo CH, Chen PS, Cho CF, Chang CF, Shi GY, Wu HL. Thrombomodulin functions as a plasminogen receptor to modulate angiogenesis. FASEB J. 2013 [Abstract]

  6. Lin WL, Chang CF, Shi CS, Shi GY, Wu HL. Recombinant lectin-like domain of thrombomodulin suppresses vascular inflammation by reducing leukocyte recruitment via interacting with Lewis Y on endothelial cells. Arterioscler Thromb Vasc Biol. 2013 [Abstract]

  7. Cho CF, Chen PK, Chang PC, Wu HL, Shi GY. Human plasminogen kringle 1-5 inhibits angiogenesis and induces thrombomodulin degradation in a protein kinase A-dependent manner. J Mol Cell Cardiol. 2013 [Abstract]

  8. Cheng TL, Wu YT, Lai CH, Kao YC, Kuo CH, Liu SL, Hsu YY, Chen PK, Cho CF, Wang KC, Lin WL, Chang BI, Chen CM, Weiler H, Shi GY, Wu HL. Thrombomodulin regulates keratinocyte differentiation and promotes wound healing. J Invest Dermatol. 2013 [Abstract]

  9. Chen JY, Tsai PJ, Tai HC, Tsai RL, Chang YT, Wang MC, Chiou YW, Yeh ML, Tang MJ, Lam CF, Shiesh SC, Li YH, Tsai WC, Chou CH, Lin LJ, Wu HL, Tsai YS. Increased aortic stiffness and attenuated lysyl oxidase activity in obesity. Arterioscler Thromb Vasc Biol. 2013 [Abstract]

  10. Lin PY, Wu YT, Lin GC, Shih YH, Sampilvanjil A, Chen LR, Yang YJ, Wu HL, Jiang MJ. Coarctation-induced degenerative abdominal aortic aneurysm in a porcine model. J Vasc Surg. 2013 [Abstract]

  11. Chao TH, Tseng SY, Li YH, Liu PY, Cho CL, Shi GY, Wu HL, Chen JH. A novel vasculo-angiogenic effect of cilostazol mediated by cross-talk between multiple signalling pathways including the ERK/p38 MAPK signalling transduction cascade. Clin Sci (Lond). 2012 [Abstract]

  12. Hsu YY, Shi GY, Kuo CH, Liu SL, Wu CM, Ma CY, Lin FY, Yang HY, Wu HL. Thrombomodulin is an ezrin-interacting protein that controls epithelial morphology and promotes collective cell migration. FASEB J. 2012 [Abstract]

  13. Ma CY, Shi GY, Shi CS, Kao YC, Lin SW, Wu HL. Monocytic thrombomodulin triggers LPS- and gram-negative bacteria-induced inflammatory response. J Immunol. 2012 [Abstract]

  14. Li YH, Kuo CH, Shi GY, Wu HL. The role of thrombomodulin lectin-like domain in inflammation. J Biomed Sci. 2012 [Abstract]

  15. Kao YC, Jiang SJ, Pan WA, Wang KC, Chen PK, Wei HJ, Chen WS, Chang BI, Shi GY, Wu HL. The epidermal growth factor-like domain of CD93 is a potent angiogenic factor. PLoS One. 2012 [Abstract]

  16. Chuang YC, Lei HY, Lin YS, Liu HS, Wu HL, Yeh TM. Dengue virus-induced autoantibodies bind to plasminogen and enhance its activation. J Immunol. 2011 [Abstract]

  17. Cheng TL, Wu YT, Lin HY, Hsu FC, Liu SK, Chang BI, Chen WS, Lai CH, Shi GY, Wu HL. Functions of rhomboid family protease RHBDL2 and thrombomodulin in wound healing. J Invest Dermatol. 2011 [Abstract]

  18. Wei HJ, Li YH, Shi GY, Liu SL, Chang PC, Kuo CH, Wu HL. Thrombomodulin domains attenuate atherosclerosis by inhibiting thrombin-induced endothelial cell activation. Cardiovasc Res. 2011 [Abstract]

  19. Lai CH, Luo CY, Lin PY, Kan CD, Chang RS, Wu HL, Yang YJ. Surgical consideration of in situ prosthetic replacement for primary infected abdominal aortic aneurysms. Eur J Vasc Endovasc Surg. 2011 [Abstract]

  20. Chang YT, Wu HL, Guo HR, Cheng YY, Tseng CC, Wang MC, Lin CY, Sung JM. Handgrip strength is an independent predictor of renal outcomes in patients with chronic kidney diseases. Nephrol Dial Transplant. 2011 [Abstract]

  21. Li YH, Chung HC, Luo CY, Chao TH, Shyu KG, Shi GY, Wu HL. Thrombomodulin is upregulated in cardiomyocytes during cardiac hypertrophy and prevents the progression of contractile dysfunction. J Card Fail. 2010 [Abstract]

  22. Kao YC, Wu LW, Shi CS, Chu CH, Huang CW, Kuo CP, Sheu HM, Shi GY, Wu HL. Downregulation of thrombomodulin, a novel target of Snail, induces tumorigenesis through epithelial-mesenchymal transition. Mol Cell Biol. 2010 [Abstract]

  23. Lin YD, Yeh ML, Yang YJ, Tsai DC, Chu TY, Shih YY, Chang MY, Liu YW, Tang AC, Chen TY, Luo CY, Chang KC, Chen JH, Wu HL, Hung TK, Hsieh PC. Intramyocardial peptide nanofiber injection improves postinfarction ventricular remodeling and efficacy of bone marrow cell therapy in pigs. Circulation. 2010 [Abstract]

  24. Su YD, Chiu KC, Chang NS, Wu HL, Chen SJ. Study of cell-biosubstrate contacts via surface plasmon polariton phase microscopy. Opt Express. 2010 [Abstract]

  25. Liu HC, Eng HL, Yang YF, Wang YH, Lin KT, Wu HL, Lin TM. Aberrant RNA splicing in RHD 7-9 exons of DEL individuals in Taiwan: a mechanism study. Biochim Biophys Acta. 2010 [Abstract]

  26. He RY, Lin CY, Su YD, Chiu KC, Chang NS, Wu HL, Chen SJ. Imaging live cell membranes via surface plasmon-enhanced fluorescence and phase microscopy. Opt Express. 2010 [Abstract]

  27. Chang PC, Wu HL, Lin HC, Wang KC, Shi GY. Human plasminogen kringle 1-5 reduces atherosclerosis and neointima formation in mice by suppressing the inflammatory signaling pathway. J Thromb Haemost. 2010 [Abstract]

  28. Chan SS, Shueh YZ, Bustamante N, Tsai SJ, Wu HL, Chen JH, Hsieh PC. Genetic fate-mapping for studying adult cardiomyocyte replenishment after myocardial injury. Methods Mol Biol. 2010 [Abstract]

  29. Liu SL, Li YH, Shi GY, Tang SH, Jiang SJ, Huang CW, Liu PY, Hong JS, Wu HL. Dextromethorphan reduces oxidative stress and inhibits atherosclerosis and neointima formation in mice. Cardiovasc Res. 2009 [Abstract]

  30. Lo IC, Lin TM, Chou LH, Liu SL, Wu LW, Shi GY, Wu HL, Jiang MJ. Ets-1 mediates platelet-derived growth factor-BB-induced thrombomodulin expression in human vascular smooth muscle cells. Cardiovasc Res. 2009 [Abstract]

  31. Kao TT, Chang WN, Wu HL, Shi GY, Fu TF. Recombinant zebrafish {gamma}-glutamyl hydrolase exhibits properties and catalytic activities comparable with those of mammalian enzyme. Drug Metab Dispos. 2009 [Abstract]

  32. Shi CS, Shi GY, Hsiao HM, Kao YC, Kuo KL, Ma CY, Kuo CH, Chang BI, Chang CF, Lin CH, Wong CH, Wu HL. Lectin-like domain of thrombomodulin binds to its specific ligand Lewis Y antigen and neutralizes lipopolysaccharide-induced inflammatory response. Blood. 2008 [Abstract]

  33. Chen YH, Huang YH, Wu HL, Wu MP, Chang WT, Kuo YZ, Lu KC, Wu HL. Angiostatin K1-3 induces E-selectin via AP1 and Ets1: a mediator for anti-angiogenic action of K1-3. J Thromb Haemost. 2008 [Abstract]

  34. Lin TM, Chen CH, Wu HL, Wang CH, Chen YL, Eng HL. The association of C (-260)-->T polymorphism in CD14 promoter and Chlamydia pneumoniae infection in ischemic stroke patients. Am J Clin Pathol. 2008 [Abstract]

  35. Liu PY, Lin CC, Tsai WC, Li YH, Lin LJ, Shi GY, Hong JS, Chen JH, Wu HL. Treatment with dextromethorphan improves endothelial function, inflammation and oxidative stress in male heavy smokers. J Thromb Haemost. 2008 [Abstract]

  36. Chang PC, Chang YJ, Wu HL, Chang CW, Lin CI, Wang WC, Shi GY. Mutation of human plasminogen kringle 1-5 enhances anti-angiogenic action via increased interaction with integrin alpha(v)beta(3). Thromb Haemost. 2008 [Abstract]

  37. Kao TT, Wang KC, Chang WN, Lin CY, Chen BH, Wu HL, Shi GY, Tsai JN, Fu TF. Characterization and comparative studies of zebrafish and human recombinant dihydrofolate reductases--inhibition by folic acid and polyphenols. Drug Metab Dispos. 2008 [Abstract]

  38. Wu HL, Lin CI, Huang YL, Chen PS, Kuo CH, Chen MS, Wu GC, Shi GY, Yang HY, Lee H. Lysophosphatidic acid stimulates thrombomodulin lectin-like domain shedding in human endothelial cells. Biochem Biophys Res Commun. 2008 [Abstract]

  39. Liu PY, Li YH, Chao TH, Wu HL, Lin LJ, Tsai LM, Chen JH. Synergistic effect of cytochrome P450 epoxygenase CYP2J2*7 polymorphism with smoking on the onset of premature myocardial infarction. Atherosclerosis. 2007 [Abstract]

  40. Li YH, Wu HL, Yang YH, Tsai HS, Chao TH. Effect of early versus late in-hospital initiation of statin therapy on the clinical outcomes of patients with acute coronary syndrome. Int Heart J. 2007 [Abstract]

  41. Li YH, Hsieh CY, Wang DL, Chung HC, Liu SL, Chao TH, Shi GY, Wu HL. Remodeling of carotid arteries is associated with increased expression of thrombomodulin in a mouse transverse aortic constriction model. Thromb Haemost. 2007 [Abstract]

  42. Liu SL, Li YH, Shi GY, Jiang MJ, Chang JH, Wu HL. The effect of statin on the aortic gene expression profiling. Int J Cardiol. 2007 [Abstract]

  43. Shi CS, Huang HC, Wu HL, Kuo CH, Chang BI, Shiao MS, Shi GY. Salvianolic acid B modulates hemostasis properties of human umbilical vein endothelial cells. Thromb Res. 2007 [Abstract]

  44. Liu SL, Li YH, Shi GY, Chen YH, Huang CW, Hong JS, Wu HL. A novel inhibitory effect of naloxone on macrophage activation and atherosclerosis formation in mice. J Am Coll Cardiol. 2006 [Abstract]

  45. Liu PY, Li YH, Chan SH, Lin LJ, Wu HL, Shi GY, Chen JH. Genotype-phenotype association of matrix metalloproteinase-3 polymorphism and its synergistic effect with smoking on the occurrence of acute coronary syndrome. Am J Cardiol. 2006 [Abstract]

  46. Liu PY, Li YH, Wu HL, Chao TH, Tsai LM, Lin LJ, Shi GY, Chen JH. Platelet-activating factor-acetylhydrolase A379V (exon 11) gene polymorphism is an independent and functional risk factor for premature myocardial infarction. J Thromb Haemost. 2006 [Abstract]

  47. Li YH, Shi GY, Wu HL. The role of thrombomodulin in atherosclerosis: from bench to bedside. Cardiovasc Hematol Agents Med Chem. 2006 [Abstract]

  48. Chen YH, Wu HL, Li C, Huang YH, Chiang CW, Wu MP, Wu LW. Anti-angiogenesis mediated by angiostatin K1-3, K1-4 and K1-4.5. Involvement of p53, FasL, AKT and mRNA deregulation. Thromb Haemost. 2006 [Abstract]

  49. Li YH, Liu SL, Shi GY, Tseng GH, Liu PY, Wu HL. Thrombomodulin plays an important role in arterial remodeling and neointima formation in mouse carotid ligation model. Thromb Haemost. 2006 [Abstract]

  50. Liu PY, Li YH, Tsai WC, Tsai LM, Chao TH, Wu HL, Chen JH. Stromelysin-1 promoter 5A/6A polymorphism is an independent genetic prognostic risk factor and interacts with smoking cessation after index premature myocardial infarction. J Thromb Haemost. 2005 [Abstract]

  51. Description Title ShortDetails [Abstract]

  52. Shi CS, Shi GY, Chang YS, Han HS, Kuo CH, Liu C, Huang HC, Chang YJ, Chen PS, Wu HL. Evidence of human thrombomodulin domain as a novel angiogenic factor. Circulation. 2005 [Abstract]

  53. Chen CH, Lo YK, Ke D, Liu CK, Liou CW, Wu HL, Lai ML; Southern Taiwan Young Stroke Study Group. Platelet glycoprotein Ia C807T, Ib C3550T, and IIIa Pl(A1/A2) polymorphisms and ischemic stroke in young Taiwanese. J Neurol Sci. 2004 [Abstract]

  54. Chao TH, Li YH, Chen JH, Wu HL, Shi GY, Liu PY, Tsai WC, Guo HR. The 161TT genotype in the exon 6 of the peroxisome-proliferator-activated receptor gamma gene is associated with premature acute myocardial infarction and increased lipid peroxidation in habitual heavy smokers. Clin Sci (Lond). 2004 [Abstract]

  55. Jiang SJ, Lin TM, Shi GY, Eng HL, Chen HY, Wu HL. Inhibition of bovine herpesvirus-4 replication in endothelial cells by arsenite. Antiviral Res. 2004 [Abstract]

  56. Jiang SJ, Lin TM, Shi GY, Eng HL, Chen HY, Wu HL. Inhibition of bovine herpesvirus-4 replication by arsenite through downregulation of the extracellular signal-regulated kinase signaling pathway. J Biomed Sci. 2004 [Abstract]

  57. Huang HC, Shi GY, Jiang SJ, Shi CS, Wu CM, Yang HY, Wu HL. Thrombomodulin-mediated cell adhesion: involvement of its lectin-like domain. J Biol Chem. 2003 [Abstract]

  58. Chen YH, Wu HL, Chen CK, Huang YH, Yang BC, Wu LW. Angiostatin antagonizes the action of VEGF-A in human endothelial cells via two distinct pathways. Biochem Biophys Res Commun. 2003 [Abstract]

  59. Liu PY, Li YH, Tsai WC, Chao TH, Tsai LM, Wu HL, Chen JH. Prognostic value and the changes of plasma levels of secretory type II phospholipase A2 in patients with coronary artery disease undergoing percutaneous coronary intervention. Eur Heart J. 2003 [Abstract]

  60. Liu PY, Chen JH, Li YH, Wu HL, Shi GY. Synergistic effect of stromelysin-1 (matrix metallo-proteinase-3) promoter 5A/6A polymorphism with smoking on the onset of young acute myocardial infarction. Thromb Haemost. 2003 [Abstract]

  61. Cheng PN, Chang TT, Jen CM, Ko AW, Young KC, Wu HL. Molecular evidence for transmission of GB virus-C/Hepatitis G virus infection within family: close relationship between mother and child. Hepatogastroenterology. 2003 [Abstract]

  62. Chang YJ, Wu HL, Hsu YC, Hamaguchi N, Shi GY, Shen MC, Lin SW. Discontinuous residues of factor IX constitute a surface for binding the anti-factor IX monoclonal antibody A-5. Thromb Res. 2003 [Abstract]

  63. Young KC, Lin PW, Hsiao WC, Chang TT, Chang YC, Wu HL. Variation of hepatitis C virus load, hypervariable region 1 quasispecies and CD81 hepatocyte expression in hepatocellular carcinoma and adjacent non-cancerous liver. J Med Virol. 2002 [Abstract]

  64. Chang YJ, Wu HL, Hamaguchi N, Hsu YC, Lin SW. Identification of functionally important residues of the epidermal growth factor-2 domain of factor IX by alanine-scanning mutagenesis. Residues Asn(89)-Gly(93) are critical for binding factor VIIIa. J Biol Chem. 2002 [Abstract]

  65. Li YH, Chen JH, Tsai WC, Chao TH, Guo HR, Tsai LM, Wu HL, Shi GY. Synergistic effect of thrombomodulin promoter -33G/A polymorphism and smoking on the onset of acute myocardial infarction. Thromb Haemost. 2002 [Abstract]

  66. Jiang SJ, Lin TM, Wu HL, Han HS, Shi GY. Decrease of fibrinolytic activity in human endothelial cells by arsenite. Thromb Res. 2002 [Abstract]

  67. Chang TT, Chang TY, Chen CC, Young KC, Roan JN, Lee YC, Cheng PN, Wu HL. Existence of hepatitis C virus in Culex quinquefasciatus after ingestion of infected blood: experimental approach to evaluating transmission by mosquitoes. J Clin Microbiol. 2001 [Abstract]

  68. Shieh SJ, Chiu HY, Shi GY, Wu CM, Wang JC, Chen CH, Wu HL. A novel platelet-rich arterial thrombosis model in rabbits. Simple, reproducible, and dynamic real-time measurement by using double-opposing inverted-sutures model. Thromb Res. 2001 [Abstract]

  69. Huang YH, Liu CC, Wang ST, Lei HY, Liu HL, Lin YS, Wu HL, Yeh TM. Activation of coagulation and fibrinolysis during dengue virus infection. J Med Virol. 2001 [Abstract]

  70. Li YH, Chen CH, Yeh PS, Lin HJ, Chang BI, Lin JC, Guo HR, Wu HL, Shi GY, Lai ML, Chen JH. Functional mutation in the promoter region of thrombomodulin gene in relation to carotid atherosclerosis. Atherosclerosis. 2001 [Abstract]

  71. Cheng PN, Chang TT, Yang MH, Hu SC, Young KC, Wu HL, Jen CM, Ko AW, Lu SC. GB virus-C/hepatitis G virus infection in a hepatitis C virus endemic village: prevalence in residents with low educational attainment and frequent recovery in females. Liver. 2000 [Abstract]

  72. Lin TM, Jiang MJ, Eng HL, Shi GY, Lai LC, Huang BJ, Huang KY, Wu HL. Experimental infection with bovine herpesvirus-4 enhances atherosclerotic process in rabbits. Lab Invest. 2000 [Abstract]

  73. Lin TM, Shi GY, Jiang SJ, Tsai CF, Hwang BJ, Hsieh CT, Wu HL. Persistent infection of bovine herpesvirus type 4 in bovine endothelial cell cultures. Vet Microbiol. 1999 [Abstract]

  74. Lin MT, Su WC, Cheng ML, Cheng KS, Chang WC, Wing LH, Jen CJ, Wu HL. The various effects of fractionated oxidized low density lipoproteins on the growth of smooth muscle cells in culture. J Biomed Sci. 1999 [Abstract]

  75. Shi GY, Chang BI, Su SW, Young KC, Wu DH, Chang LC, Tsai YS, Wu HL. Preparation of a novel streptokinase mutant with improved stability. Thromb Haemost. 1998 [Abstract]

  76. Young KC, Shi GY, Wu DH, Chang LC, Chang BI, Ou CP, Wu HL. Plasminogen activation by streptokinase via a unique mechanism. J Biol Chem. 1998 [Abstract]

  77. Huang YH, Chang BI, Lei HY, Liu HS, Liu CC, Wu HL, Yeh TM. Antibodies against dengue virus E protein peptide bind to human plasminogen and inhibit plasmin activity. Clin Exp Immunol. 1997 [Abstract]

  78. Lin TM, Shi GY, Tsai CF, Su HJ, Guo YL, Wu HL. Susceptibility of endothelial cells to bovine herpesvirus type 4 (BHV-4). J Virol Methods. 1997 [Abstract]

  79. Chang TT, Young KC, Yang YJ, Lai KA, Wu HL, Wu MH, Chen MY, Lin XZ, Lin CY, Shin JS. Incidence of post-transfusion hepatitis in Taiwan before and after introduction of anti-HCV testing. Liver. 1996 [Abstract]

  80. Chang TT, Young KC, Yang YJ, Lei HY, Wu HL. Hepatitis C virus RNA in peripheral blood mononuclear cells: comparing acute and chronic hepatitis C virus infection. Hepatology. 1996 [Abstract]

  81. Chang WC, Shyu WJ, Shi GY, Lin MT, Jen CJ, Wing LY, Tang MJ, Wu HL. Characterization of Phospholipase A(2) Activation by Plasmin in Cultured Bovine Endothelial Cells. J Biomed Sci. 1996 [Abstract]

  82. Shi GY, Wang SJ, Chang B, Tasi CF, Lin MT, Chang WC, Wing LY, Jen CJ, Wu HL. Regulation of plasminogen activator inhibitor activity by plasmin in endothelial cells. Thromb Res. 1996 [Abstract]

  83. Young KC, Shi GY, Chang YF, Chang BI, Chang LC, Lai MD, Chuang WJ, Wu HL. Interaction of streptokinase and plasminogen. Studied with truncated streptokinase peptides. J Biol Chem. 1995 [Abstract]

  84. Shi GY, Chang BI, Chen SM, Wu DH, Wu HL. Function of streptokinase fragments in plasminogen activation. Biochem J. 1994 [Abstract]

  85. Chang WC, Shi GY, Lin MT, Jen CJ, Wing LY, Wu HL. Diacylglycerol formation induced by human plasmin in cultured endothelial cells. Thromb Res. 1994 [Abstract]

  86. Kuo PL, Lin TM, Huang KF, Yeh NG, Wu DB, Wu RC, Tzeng CC, Wu HL, Huang KE. Carrier screening and prenatal diagnosis for alpha-thalassemia with biphasic polymerase chain reaction. J Formos Med Assoc. 1994 [Abstract]

  87. Jen CJ, Huang TY, Chen HI, Wing LY, Lin MT, Wu HL, Chang WC. Regional differences in prostaglandin production rates among porcine intrathoracic vessels. Prostaglandins. 1994 [Abstract]

  88. Chang TT, Liou TC, Young KC, Lin XZ, Lin CY, Shin JS, Wu HL. Intrafamilial transmission of hepatitis C virus: the important role of inapparent transmission. J Med Virol. 1994 [Abstract]

  89. Wu HL, Yang WH, Wang MY, Shi GY. Impaired fibrinolysis in patients with Blackfoot disease. Thromb Res. 1993 [Abstract]

  90. Young KC, Chang TT, Liou TC, Wu HL. Detection of hepatitis C virus RNA in peripheral blood mononuclear cells and in saliva. J Med Virol. 1993 [Abstract]

  91. Shi GY, Chang BI, Wu DH, Wu HL. Interaction of immobilized human plasminogen and plasmin with streptokinase. Biochem Biophys Res Commun. 1993 [Abstract]

  92. Chang TT, Lin H, Yen YS, Wu HL. Hepatitis B and hepatitis C among institutionalized psychiatric patients in Taiwan. J Med Virol. 1993 [Abstract]

  93. Chang WC, Shi GY, Chow YH, Chang LC, Hau JS, Lin MT, Jen CJ, Wing LY, Wu HL. Human plasmin induces a receptor-mediated arachidonate release coupled with G proteins in endothelial cells. Am J Physiol. 1993 [Abstract]

  94. Lin TM, Eng HL, Kuo PL, Wu HL. Neonatal screening for alpha-thalassemia in southern Taiwan. J Formos Med Assoc. 1992 [Abstract]

  95. Wu HL, Wu IS, Fang RY, Hau JS, Wu DH, Chang BI, Lin TM, Shi GY. The binding of plasminogen fragments to cultured human umbilical vein endothelial cells. Biochem Biophys Res Commun. 1992 [Abstract]

  96. Liou TC, Chang TT, Young KC, Lin XZ, Lin CY, Wu HL. Detection of HCV RNA in saliva, urine, seminal fluid, and ascites. J Med Virol. 1992 [Abstract]

  97. Chen SH, Wu HL, Lin MT, Jen CJ, Wing LY, Lei HY, Tsao CJ, Chang WC. Cytoprotective effect of reduced glutathione in hydrogen peroxide-induced endothelial cell injury. Prostaglandins Leukot Essent Fatty Acids. 1992 [Abstract]

  98. Shi GY, Wu DH, Wu HL. Kringle domains and plasmin denaturation. Biochem Biophys Res Commun. 1991 [Abstract]

  99. Chang WC, Chen SH, Wu HL, Shi GY, Murota S, Morita I. Cytoprotective effect of reduced glutathione in arsenical-induced endothelial cell injury. Toxicology. 1991 [Abstract]

  100. Wu HL, Chang BI, Wu DH, Chang LC, Gong CC, Lou KL, Shi GY. Interaction of plasminogen and fibrin in plasminogen activation. J Biol Chem. 1990 [Abstract]

  101. Chang WC, Wu HL, Hsu SY, Chen FS. Isolation of rat renal NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase. Prostaglandins Leukot Essent Fatty Acids. 1990 [Abstract]

  102. Shi GY, Change BI, Wu DH, Ha YM, Wu HL. Activation of human and bovine plasminogens by the microplasmin and streptokinase complex. Thromb Res. 1990 [Abstract]

  103. Shi GY, Wu HL. Isolation and characterization of microplasminogen. A low molecular weight form of plasminogen. J Biol Chem. 1988 [Abstract]

  104. Shi GY, Wu HL. Differential autolysis of human plasmin at various pH levels. Thromb Res. 1988 [Abstract]

  105. Wu HL, Shi GY, Wohl RC, Bender ML. Structure and formation of microplasmin. Proc Natl Acad Sci U S A. 1987 [Abstract]

  106. Wu HL, Shi GY, Bender ML. Preparation and purification of microplasmin. Proc Natl Acad Sci U S A. 1987 [Abstract]

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Updated: 2014.11.20