成功大學醫學院生化所
鄭文義 助理研究員

Dr. Wen-Yih Jeng

學歷
成功大學基礎醫學所 博士 (2004)
成功大學醫事技術學系 學士 (1992-1996)　

研究方向
利用蛋白質結晶學技術進行蛋白質三維結構解析，從原子的角度探索分子間的交互作用機制。

研究興趣

• 細菌的致病因子或多重抗性蛋白：細菌的致病因子或多重抗性蛋白與細菌的致病能力及抵抗外在環境的能力息息相關，能夠清楚地了解這些蛋白質的作用機轉，將有助於新藥物的開發或治療策略的提供。
　　
• 纖維素水解酵素功能性之改良：纖維素水解酵素的三維結構解析，將可以提供酵素的結構資訊用於酵素功能的改良。
　　
• 藥用天然物之生合成酵素：解析藥用天然物生合成酵素之三維結構，將有助於後續利用基因工程法進行藥用天然物之生產。

Publications:

1. Liu WC, Lin YS, Jeng WY, Chen JH, Wang AH, Shyur LF.(2012) Engineering of dual-functional hybrid glucanases. Protein Eng Des Sel. 2012 Oct 18.[Epub ahead of print] PMID:23081838 [Abstract]


2. Lee HL, Chang CK, Jeng WY, Wang AH, Liang PH.(2012) Mutations in the substrate entrance region of β-glucosidase from Trichoderma reesei improve enzyme activity and thermostability.Protein Eng Des Sel. 2012 Oct 16.[Epub ahead of print] PMID:23077275 [Abstract]


3. Chang YM, Chen CK, Chang YC, Jeng WY, Hou MH, Wang AH.(2012) Functional Studies of ssDNA Binding Ability of MarR Family Protein TcaR from Staphylococcus epidermidis.PLoS One. 2012;7(9):e45665.doi: 10.1371/journal.pone.0045665.Epub 2012 Sep 21. PMID:23029170 [Abstract]


4. Jeng WY, Wang NC, Lin CT, Chang WJ, Liu CI, Wang AH.(2012) High-resolution structures of Neotermes koshunensis beta-glucosidase mutants provide insights into the catalytic mechanism and the synthesis of glucoconjugates.Acta Crystallogr D Biol Crystallogr. 2012 Jul;68(Pt 7):829-38.doi: 10.1107/S0907444912013224.Epub 2012 Jun 19.PMID: 22751668 [Abstract]


5. Liu CI, Jeng WY, Chang WJ, Ko TP, Wang AH.(2012) Binding modes of zaragozic acid A to human squalene synthase and staphylococcal dehydrosqualene synthase.J Biol Chem. 2012 May 25;287(22):18750-7.Epub 2012 Apr 3.PMID: 22474324 [Abstract]


6. Jeng, W.Y., Wang, N.C., Lin, C.T., Shyur, L.F., and Wang, A.H.J.(2011) Crystal structures of the laminarinase catalytic domain from Thermotoga maritima MSB8 in complex with inhibitors: essential residues for β-1,3 and β-1,4 glucan selection.J Biol Chem. 2011 Dec 30; 286, 45030-45040.(Epub 2011 Nov 7.) [Abstract]


7. Jeng, W.Y., Wang, N.C., Lin, M.H., Lin, C.T., Liaw, Y.C., Chang, W.J., Liu, C.I., Liang, P.H., and Wang, A.H.J.(2011) Structural and functional analysis of three-glucosidases from bacterium Clostridium cellulovorans, fungus Trichoderma reesei and termite Neotermes koshunensis, J Struct Biol. 173, 46-56.(Epub 2010 Aug 1) (Featured on cover) [Abstract]


8. Lin, F.Y., Liu, C.I, Liu, Y.L., Zhang, Y., Wang, K., Jeng, W.Y., Ko, T.P., Cao, R., Wang, A.H., Oldfield, E.(2010) Mechanism of action and inhibition of dehydrosqualene synthase, Proc Natl Acad Sci U S A 107, 21337-21342. [Abstract]


9. Chang, S.H., Han, J.L., Tseng, S.Y., Lee, Hsin.Y., Lin, C.W., Lin, Y.C., Jeng, W.Y., Wang, A.H.-J., Wu, C.Y.and Wong C.H.(2010) Glycan Array on Aluminum Oxide Coated Glass Slides through Phosphonate Chemistry, J Am Chem Soc. 132, 13371-13380. [Abstract]


10. Chang, Y.M., Jeng, W.Y., Ko, T.P., Yeh, Y.J., Chen, C.K., and Wang, A.H.J.(2010) Structural study of TcaR and its complexes with multiple antibiotics from Staphylococcus epidermidis, Proc Natl Acad Sci U S A 107, 8617-8622. [Abstract]


11. Ko, T.P.*, Jeng, W.Y.*, Liu, C.I., Lai, M.D., Wu, C.L., Chang, W.J., Shr, H.L., Lu, T.J., and Wang, A.H.J.(2010) Structures of human MST3 kinase in complex with adenine, ADP and Mn2+, Acta Crystallogr D Biol Crystallogr 66, 145-154.(* These authors contributed equally.) [Abstract]


12. Song, Y.C., Liu, C.I., Lin, F.Y., No, J.H., Hensler, M., Liu, Y.L., Jeng, W.Y., Low, J., Liu, G.Y., Nizet, V., Wang, A.H.J., and Oldfield, E.(2009) Inhibition of Staphyloxanthin Virulence Factor Biosynthesis in Staphylococcus aureus: In Vitro, in Vivo, and Crystallographic Results, J Med Chem 52, 3869-3880. [Abstract]


13. Chen, C.Y., Shiu, J.H., Hsieh, Y.H., Liu, Y.C., Chen, Y.C., Chen, Y.C., Jeng, W.Y., Tang, M.J., Lo, S.J., and Chuang, W.J.(2009) Effect of D To E mutation of the RGD motif in rhodostomin on its activity, structure, and dynamics: Importance of the interactions between the D residue and integrin, Proteins 76, 808-821. [Abstract]


14. Liu, C.I., Liu, G.Y., Song, Y.C., Yin, F.L., Hensler, M.E., Jeng, W.Y., Nizet, V., Wang, A.H.J., and Oldfield, E.(2008) A cholesterol biosynthesis inhibitor blocks Staphylococcus aureus virulence, Science 319, 1391-1394. [Abstract]


15. Jeng, W.Y., Ko, T.P., Liu, C.I., Guo, R.T., Liu, C.L., Shr, H.L., and Wang, A.H.J.(2008) Crystal structure of IcaR, a repressor of the TetR family implicated in biofilm formation in Staphylococcus epidermidis, Nucleic Acids Res 36, 1567-1577.(Featured on cover) [Abstract]


16. Guo, R.T., Cao, R., Liang, P.H., Ko, T.P., Chang, T.H., Hudock, M.P., Jeng, W.Y., Chen, C.K.M., Zhang, Y.H., Song, Y.C., Kuo, C.J., Yin, F.L., Oldfield, E., and Wang, A.H.J.(2007) Bisphosphonates target multiple sites in both cis- and trans-prenyltransferases, Proc Natl Acad Sci USA 104, 10022-10027. [Abstract]


17. Anangi, R., Chen, C.Y., Cheng, C.H., Chen, Y.C., Chen, C.C., Chu, Y.P., Chang, C.H., Jeng, W.Y., Shiu, J.H., and Chuang, W.J.(2007) Expression of snake venom toxins in Pichia pastoris, Toxin Rev 26, 169-187. [Abstract]


18. Lu, T.J., Huang, C.Y.F., Yuan, C.J., Lee, Y.C., Leu, T.H., Chang, W.C., Lu, T.L., Jeng, W.Y., and Lai, M.D.(2005) Zinc ion acts as a cofactor for serine/threonine kinase MST3 and has a distinct role in autophosphorylation of MST3, J Inorg Biochem 99, 1306-1313. [Abstract]


19. Jeng, W.Y., Tsai, Y.H., and Chuang, W.J.(2004) The catalase activity of N�-acetyl-microperoxidase-8, J Pept Res 64, 104-109. [Abstract]


20. Luo, S.C., Chen, C.Y., Lin, Y.S., Jeng, W.Y., and Chuang, W.J.(2003) Backbone H-1, N-15 and C-13 resonance assignments of the 28 kDa mature form of streptopain, J Biomol Nmr 25, 165-166. [Abstract]


21. Chen, C.Y., Luo, S.C., Kuo, C.F., Lin, Y.S., Wu, J.J., Lin, M.T., Liu, C.C., Jeng, W.Y., and Chuang, W.J.(2003) Maturation processing and characterization of streptopain, J Biol Chem 278, 17336-17343. [Abstract]


22. Liu, P.P., Chen, Y.C., Li, C., Hsieh, Y., Chen, S.W., Chen, S.H., Jeng, W.Y., and Chuang, W.J.(2002) Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a), Proteins 49, 543-553. [Abstract]


23. Jeng, W.Y., Chen, C.Y., Chang, H.C., and Chuang, W.J.(2002) Expression and characterization of recombinant human cytochrome c in E.coli, J Bioenerg Biomembr 34, 423-431. [Abstract]


24. Chuang, W.J., Yeh, I.J., Hsieh, Y.H., Liu, P.P., Chen, S.W., and Jeng, W.Y. (2002) Letter to the Editor: H-1, N-15 and C-13 resonance assignments for the DNA-binding domain of myocyte nuclear factor (Foxk1), J Biomol Nmr 24, 75-76. [Abstract]


25. Chuang, W.J., Chang, Y.D., and Jeng, W.Y. (1999) Kinetic and structural studies of N�-acetyl-microperoxidase-5 and -microperoxidase-8, J Inorg Biochem 75, 93-97. [Abstract(cited from Web of Science)]